Interaction Between Serum/Glucocorticoid-Regulated Kinase 1 and Interleukin-6 in Chronic Rhinosinusitis

PURPOSE Serum/glucocorticoid-regulated kinase 1 (SGK1) has recently emerged as a critical regulator of inflammatory diseases. In this study, we examined SGK1 expression and its possible pathogenic roles in chronic rhinosinusitis (CRS). METHODS Immunohistochemistry, western blotting, Bio-Plex assay, enzyme-linked immunosorbent assays, quantitative real-time polymerase chain reaction were performed to assess protein gene levels. The mRNA levels interleukin-6 (IL-6) extracted from CRS database perform correlation analysis. Stable cell lines with overexpression (16HBE) knockdown (A549) constructed investigate the interaction between IL-6 vitro. RESULTS exhibited strong cytoplasmic nuclear staining epithelial layers lamina propria nasal polyps (NPs) mucosal tissues without (CRSsNP). significantly increased NPs CRSsNP tissues, compared control tissues. phosphorylation was greater than (P < 0.01). correlated 0.001, r = 0.649). Exposure cultured dispersed NP cells, 16HBE A549 cells. down-regulated SGK1-overexpressing cells 0.01) up-regulated SGK1-knockdown 0.05). Administration GSK650394, inhibitor, self-induced CONCLUSIONS may play an anti-inflammatory role IL-6-induced inflammation pathogenesis CRS.